FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3305137
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14250.033.6223871
TCTGTCG15100.030.9903748
TCGCTCT14800.029.6728024
GTACTAG8400.028.5189911
GTCTTGC32250.027.9120311
ACCGTGT11600.027.30778
TTCACCG10400.026.3051435
GTGTTAC16000.024.9541191
TCTCGCT17150.024.7849042
GTATAAT10400.024.4306011
CGCTCTG17500.024.2719365
CTCGCTC17650.023.6763943
CTGTCGC19700.023.3885969
GTATTGG11400.022.9243551
GTACTAT9250.022.7591341
GTATTAG13300.022.3785381
GTTACCC12600.022.30113
GTTTCAC54700.022.2603323
TTGCTCT44000.022.088774
CCGTGTT12450.021.9737749
Produced by FastQC (version 0.11.5)