FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3305137
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14250.041.0888561
TCTGTCG15600.038.76518
TCGCTCT17400.034.7575454
CGCTCTG18900.031.2361835
CTCGCTC19050.030.2348293
TCTCGCT19300.029.8458942
CTGTCGC21950.028.8620919
GTCTTGC33550.028.5777261
GTATTAG12300.025.5865821
GTCTTAC15200.024.5570091
GTCTCAC39350.023.0635361
GTATTAA9800.022.4049571
TTGCTCT45750.022.1894784
ATACCGC1952.7430517E-422.1524936
GTACTAT9300.022.0355571
GTATAGT10100.021.7394641
TCTACGC2704.726877E-621.332353
CTTGCTC44100.021.223513
GGTATAC7950.021.1743261
GTGTTAG25650.021.1151051
Produced by FastQC (version 0.11.5)