FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2917558
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12900.043.531888
CTGTCGC15750.036.1117979
GTCTCGC14100.030.8748381
GTCTTGC31250.030.183241
TCGCTCT14150.029.0015374
GTATTAG10250.028.3144841
TCTCGCT17100.025.700062
TAATACT11000.025.5255154
GGTATAC7350.024.6788651
CTCGCTC14750.024.4235153
GTGTAAT14550.023.9359551
GTGTTAG21150.023.3276541
GTACTAC5650.023.1151431
GCGTTAC1952.602804E-422.3248831
TTGCTCT40000.022.1384754
GTGTTAC15450.022.0720121
GTTTCAC47050.022.0512433
GGGTTTC54750.021.866151
TTCACCG10250.021.7741095
GTGTTAT14350.021.741481
Produced by FastQC (version 0.11.5)