FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3890937
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC17150.038.4918751
TCTGTCG15900.038.4879658
TCGCTCT18050.035.4989854
TCTCGCT19750.032.462572
CTCGCTC19700.031.0846653
CGCTCTG20700.029.9110225
CTGTCGC20700.029.563229
GTATTAT13100.025.4728971
GTCTTGC37200.025.3508321
GTCTTAC14200.024.0105081
GTGTAAA23100.023.5527211
GTATAAT10650.023.1590711
GTGTTAG27650.021.7758331
GGAGTGC84500.021.470745
GTCTCAC42750.021.3809131
GTATTGG14800.021.0765041
GAGTGCA91950.021.0621996
GTGTAAG19800.020.5170381
GTATTAC9300.020.2806641
TAGTACT10100.019.9590474
Produced by FastQC (version 0.11.5)