FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299299
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13750.038.2228748
GTCTCGC15550.035.432751
GTCTTGC32900.033.9348951
GTATTAT9750.031.9731561
CTGTCGC18350.030.9951829
TCGCTCT16800.029.9979744
TCTCGCT18000.029.2161832
GGTATAC7450.028.2204151
TACACTG20900.027.5579475
GGGTACG3453.6379788E-1227.3178141
GTCTTAC14050.026.3157371
GTACCGA1400.001000165425.8918841
CTCGCTC19550.025.0592563
GTGTTAG23850.024.9256881
TTACACT15350.023.9202064
TTGCTCT46100.023.581924
CGCTCTG18350.023.5406445
GATCGTT4403.6379788E-1222.9075439
GTATAAT9550.022.7740141
CGGTACT1902.3009875E-422.7353064
Produced by FastQC (version 0.11.5)