FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299299
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15800.039.8072781
TCGCTCT17300.034.9564174
GTCGTAC1301.5600817E-533.7542951
TCTGTCG15250.032.5731548
GTCTTGC33700.031.4672811
CGCTCTG19350.029.7643345
CTCGCTC19300.029.095853
TCTCGCT22050.026.1224942
GTACTAT8000.025.5970061
CTGTCGC23200.023.582679
TGTACCG2452.034887E-623.507755
GTATAAA14050.022.903271
GTACCGT1902.301562E-422.7344686
GTATAAG11600.022.6968541
GTATTAG11350.022.5524271
TATACTG11450.022.0064355
GTATTAT11300.022.0050131
GTCTTAC14650.021.9652541
GTTTCAC48400.021.7170683
GTGTAAC11800.021.6923771
Produced by FastQC (version 0.11.5)