FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3195959
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13950.042.3187458
GTCTCGC15150.036.8722651
GTACTAG5300.035.5892871
TCGCTCT16050.035.4360434
GTCTTGC31650.033.236561
CTGTCGC18350.030.602139
TCTCGCT18100.028.6548792
CTCGCTC18700.027.3519653
CGCTCTG19050.025.6984585
GTACTAT7550.024.9832061
GTTAGCC15800.024.6211573
TAACGCA1500.001557283223.9978264
GTATTAG12850.023.1474151
TAATACG2502.425164E-623.037914
GTACGCC2202.343375E-522.9217193
GTGTTAC14350.022.7500511
CGTTTCG1902.301325E-422.7347839
GGTATAC8300.022.7256871
GTGTTAG24000.022.6710591
GTATTAT12500.022.6347831
Produced by FastQC (version 0.11.5)