FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3195959
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16000.033.297328
GTCTCGC14600.032.590741
TCGCTCT15900.031.6970544
GTCTTGC30050.031.1816561
TCTCGCT17650.026.5159382
CTGTCGC22350.025.769749
CTCGCTC18050.025.1293533
GTATTAC8400.024.4012721
GTCCTAC10800.024.4012721
GTATAAC6300.024.4012721
GTATTAG10900.024.1774081
GTATAGT9550.023.7624931
GTCTCAC39050.023.620181
CGCTCTG18600.022.8365155
GTATAGC7900.022.2391341
GTACTAT7250.022.2135721
TTGCTCT41900.021.8226594
GCATACG3054.873036E-721.6011261
GGGTTTC55050.021.409291
GTGTAAT15250.021.1210981
Produced by FastQC (version 0.11.5)