FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3682117
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT17750.040.155724
GTCTCGC17550.036.940781
GTCTTGC35750.034.657171
TCTGTCG17900.032.981138
CGCTCTG21850.031.6318455
CTCGCTC20800.030.4604193
GGTATAC7750.028.8138081
TCTCGCT22000.028.8044192
CTGTCGC21250.028.1205569
TTACACT18200.025.7129924
GTATTAG12700.025.5240441
GTATTAC8900.025.0906771
TTGCTCT48350.025.0163214
GTCTTAC16000.024.761871
CTGTTGC39500.023.5124239
GGGTTTC61200.022.9521771
GTTTCAC57100.022.9483683
GTGTTAC16800.022.725181
TTACTCT23700.021.8723164
GTAATAC8600.021.7666423
Produced by FastQC (version 0.11.5)