FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3367515
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14050.044.0659528
GTCTCGC15500.043.688531
TCGCTCT17600.038.859034
TCTCGCT17350.038.1826552
CTGTCGC17550.035.277879
GTCTTGC29200.035.1602861
CGCTCTG18600.032.8993265
CTCGCTC20100.031.883923
GTCTTAC13050.030.6879831
TTGCTCT41800.027.5565434
GTATTAG13300.026.8262881
GTTTCAC42950.025.3157943
GTATTAA9800.025.2620741
GTATTAC7600.024.9101281
GGGTTTC50450.024.6803361
GGTATAC5750.024.060351
GTGTAAC9100.024.0046861
GTCTCAC38750.023.8642711
CTTGCTC41000.023.0072783
TATACTG9150.022.8169695
Produced by FastQC (version 0.11.5)