FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3367515
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14400.048.4971288
GTCTCGC15050.043.0820731
TCGCTCT18100.038.583924
GTCTTGC30900.038.469451
CGCTCTG19350.035.718815
CTGTCGC20600.035.6494339
CTCGCTC20350.033.2579883
TCTCGCT19950.032.4869962
GTCTCAC37250.029.590731
TTGCTCT38750.027.6839314
TCTGTCA41750.026.0391758
GTTTCAC46750.026.0278113
TTCACCG8550.025.261665
GGTATAC5450.025.1158391
GGGTTTC52600.025.064321
GTATTAA10200.024.7205681
ACCGTGT9350.024.640258
TAATACT8700.023.9989344
TCACTCT44950.023.8654584
GTGTTAG23050.023.7538091
Produced by FastQC (version 0.11.5)