FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4092303
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG18050.033.1040538
GTCTCGC17350.030.6490541
TCGCTCT19600.026.8131074
CTGTCGC24200.026.4761569
GTCTTGC35500.026.2648741
GTCTCAC44850.025.1746221
TCTCGCT21550.024.3958032
CTCGCTC22050.022.8627763
CGCTCTG20600.022.715725
GTATAAG12450.021.063321
GTGTAAC11100.021.0000671
GTATCGG2154.90087E-420.3285541
GTCTTAC13750.020.1314281
GTGTTAC14750.019.75431
GTGTAAA22600.019.6614281
TCACTCT53500.019.3771114
GTGCTAC13550.018.8157881
GTATTAG13000.018.4911651
TTACACT18300.018.489584
GTATTAC7950.018.325531
Produced by FastQC (version 0.11.5)