FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4133807
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17500.039.0807088
GTCTCGC17250.036.3109251
CTGTCGC21900.032.215059
GTATAAT13450.029.2414861
TCGCTCT19450.027.3898074
GTCTTGC40500.026.6155031
TCTCGCT21500.026.462672
GTATTAC9950.026.3516251
GTATTAT15650.025.5962491
GTGTAAA27250.025.1240311
TAGTACT9800.024.976394
GTATTAA13750.023.836241
GTCTTAC17400.023.4404931
CTCGCTC22250.023.3049133
GTACCGA2202.1533293E-523.1741261
GGGTTTC74150.022.2968041
GTTTCAC66900.022.2837983
TAATACT15900.022.1858374
CGCTCTG22500.022.0771715
GTATTAG16550.021.5638371
Produced by FastQC (version 0.11.5)