FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4056836
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17200.040.5995988
CTGTCGC23050.033.1065069
GTCTTGC39400.032.5232051
GTCTCGC18400.032.0511861
TTCACCG12350.027.39745
TCGCTCT21350.027.312764
GTATTAG16550.026.835441
GTGTAAA24350.025.71441
GTGTTAC18200.025.6026841
GGGTTTC70600.025.2661041
GTTTCAC65650.024.6775023
TCACCGT14350.024.5823066
TTGCTCT52850.024.3829634
TCTCGCT23350.024.3609142
ACCGTGT14200.024.3358
GTATAAT13450.023.818111
CGCTCTG22750.023.733315
GTACGGT3452.8303475E-923.2140271
GTGTAAC12600.023.1135331
TAACACG8550.022.7340134
Produced by FastQC (version 0.11.5)