FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4056836
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG18300.045.6372958
CTGTCGC22200.039.241489
GTCTCGC19150.036.111071
TCGCTCT20250.031.6430264
GTCTTGC37550.030.8854431
CTCGCTC22050.028.733763
TCTCGCT24750.027.0593362
CGCTCTG24700.025.650675
GTATTAC10150.025.5490251
GTGTAAG22050.025.4814381
GTGTAAC12400.024.3986341
GTATACG3001.8315404E-824.0113531
GTATTAG16200.023.5666981
TACCCCG5250.023.313245
GTCTTAC17550.022.9852281
GTATTAA12600.022.8679561
GTTTCAC61900.022.5646953
GTGTAAA26700.022.392611
GTCTCAC49950.022.0644861
GTGTTAG27900.021.6876741
Produced by FastQC (version 0.11.5)