FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3433996
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14300.045.309718
GTCTCGC14950.042.8552251
TCGCTCT15900.041.655814
CTGTCGC18300.038.9465459
TCTCGCT17250.037.1561972
GTCTTGC33200.034.8675421
CTCGCTC17850.034.6968883
CGCTCTG18650.032.8114175
GTATTAC9600.027.3019451
GGTATAC7000.027.0419251
GGGTTTC57400.026.1286891
TCCGTCG1400.001041824625.7114648
GTCTCAC37800.025.4240321
GTCTTAC15250.025.3027841
GTGTTAG24200.024.6695231
TTGCTCT46900.024.4067044
GTTTCAC52250.024.1202643
GTATAAA13950.024.0074421
GTATTAG11350.023.7338491
GTATTAT11350.023.0923921
Produced by FastQC (version 0.11.5)