FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3433996
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15800.046.478198
CTGTCGC21300.039.8849039
GTCTCGC13950.038.7248081
GTATTAC8200.036.8924641
TCGCTCT16700.036.2138984
GTCTTGC31300.035.668891
CGCTCTG19050.031.3681775
TCTCGCT18050.028.7226622
CTCGCTC19350.028.2777653
TCCGTCG1555.7723828E-527.8692768
GTCTCAC40300.027.703131
TTCACCG9300.027.0951335
TTGCTCT42750.026.9461254
CCGTGTT10500.026.7412349
GTTTCAC50400.025.2845483
GTATTAG12100.025.0015051
GTATAGC7300.024.6671731
GGGTTTC54200.024.585251
CTTGCTC41500.024.114553
GTGTAGG22800.023.693471
Produced by FastQC (version 0.11.5)