FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3282093
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12800.045.556498
GTCTCGC14000.039.539561
GTCTTGC28650.035.083321
CTGTCGC16600.034.260549
TCGCTCT15200.034.1007654
TCTCGCT17100.031.1647012
CGTTTCG1753.9913048E-628.7962029
CGCTCTG19000.028.0384085
GTCGTAC1050.00753890627.7470571
CTCGCTC18900.027.4345653
GTATTAC7750.027.2547721
GGCGTAC1658.1634425E-526.4858281
GTGTTAG23000.026.2843061
GTGTAAC11150.026.1295171
GGGTTTC52700.024.1865351
GTCTCAC39700.024.0340561
TTGCTCT42900.023.8290234
GTTTCAC48450.023.6336463
GTGTAAT14400.023.2670631
GTATTAA10250.022.7390521
Produced by FastQC (version 0.11.5)