FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3602490
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14600.047.830568
GTCTCGC16400.043.0680431
TCGCTCT16400.040.385974
CTGTCGC19200.036.3711559
TCTCGCT19750.034.6383822
GTCTTGC33600.034.4576071
CTCGCTC19500.033.975073
CGCTCTG18650.032.039515
GGTATAC8500.028.269771
GTTTCAC52300.026.987433
GGGTTTC58350.026.20631
TTGCTCT46550.025.5182484
GTCTTAC13850.025.2358951
GTACTAG7450.024.4349271
GTATAAT10750.024.384921
CTCGATC14100.023.9974638
GTGTTAC16500.023.3894061
TCTCGAT14800.023.3488857
TAATACT11150.023.2441794
GTATTAC8850.023.0378551
Produced by FastQC (version 0.11.5)