FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3602490
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC16400.043.482341
TCGCTCT17400.040.1364564
TCTGTCG18600.039.0953378
GTCTTGC35000.037.45631
CGCTCTG19500.033.5987665
TCTCGCT20900.032.7324332
CTGTCGC23150.032.3443879
CTCGCTC21550.031.4053133
GTATTAG13400.030.1026541
CGTTTCG1804.9660484E-627.9989729
GTCTCAC38350.027.9861031
GTTAGCC18600.027.870333
TTGCTCT49000.026.8888074
GGGTTTC56150.026.683031
GTATTAA10650.026.3776781
GTTTCAC51900.026.3577793
GTGTTAG24250.025.8421761
TAGTCCG1400.001041390825.713345
GTGTTAC16850.024.7941721
TGTTAGC22150.024.3830742
Produced by FastQC (version 0.11.5)