FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3290258
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13550.043.566428
GTCTCGC13200.036.9564061
CTGTCGC16750.035.673089
GTCTTGC30950.035.5226331
TCGCTCT16050.033.1920434
CGCTCTG16800.028.7106385
TCTCGCT15850.028.623362
CTCGCTC16650.027.6809923
TAATACT11000.026.8329544
GTTTCAC51950.026.6153143
GGGTTTC56800.025.7653831
GTCTTAC13550.024.717661
ACCGTGT9800.024.241828
ACCATGT42550.023.6867668
GTCTCAC38450.023.480871
TTGCTCT45000.023.3570334
TAACACT18200.023.3376964
GGTTTCA55250.023.2004852
CTTGCTC41400.022.9609323
GTATTAT11750.022.9272881
Produced by FastQC (version 0.11.5)