FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3290258
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14700.037.2444231
TCTGTCG14950.033.228868
GTCTTGC31200.032.7867771
TCGCTCT16450.031.074674
CTCGCTC17850.029.4441343
CGCTCTG17700.027.6594285
TCTCGCT18800.027.5804252
GTGTAAC9800.027.1982291
CTGTCGC19450.027.0215829
GTTACAC11100.025.2962273
GTCTCAC37400.024.8475251
GTCTTAC13200.024.5585981
TAATACT9500.024.2516044
GTGTTAC16050.024.237271
GTTAGCC17850.023.393973
GTGTTAG22650.023.2177261
GTGTAAG18050.023.1481231
TTGCTCT44650.022.5746334
GTATAAT10850.022.5742931
TTACACT18000.022.3990524
Produced by FastQC (version 0.11.5)