FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3355231
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14500.039.2227638
GTCTTGC31350.036.2386361
GTCTCGC14000.031.7312071
CTGTCGC19400.031.1714259
TCGCTCT16000.031.0461854
TACACCG2151.9417712E-830.135835
GTGTAAC9350.027.2609651
GGTATAC5550.026.2435021
TCTCGCT16650.025.947012
ACCGTGT10750.025.4480328
TTGCTCT42950.025.3100414
CGCTCTG17800.025.075575
TACTACG1751.3166663E-424.6867282
CTCGCTC17800.024.2706583
GGGTTAC10050.023.9129221
GTTTCAC48500.023.902013
CTTGCTC40350.023.7333153
GTCTCAC39900.023.3626351
GTACGGA2852.665074E-722.9975951
TCTTGCT46100.022.6473482
Produced by FastQC (version 0.11.5)