FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3355231
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC16000.044.1189841
TCTGTCG16250.036.774228
TCGCTCT17700.036.2022444
TCTCGCT19850.033.3741952
GTCTTGC32100.032.0885771
CTCGCTC19350.031.9989873
CTGTCGC20800.031.499949
CGCTCTG19700.030.3340645
GTGTTAC12300.026.3528061
GTTTCAC46350.024.8535823
GGTATAC5250.024.6963421
TTGCTCT40900.024.6446974
GTCTCAC38950.024.2260881
GTGTTAG22550.023.6376671
GGGTTTC53050.023.2182871
GTCTTAC12800.023.0724281
GTGTAAC10150.022.7092781
TTACTCT19100.022.2401354
GTACTAA7150.022.1633831
GTGTAAA17900.022.1324311
Produced by FastQC (version 0.11.5)