FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037610
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8900.042.873268
GTCTTGC18350.040.8316041
GTCTCGC10000.036.0221141
CTGTCGC10850.033.1797339
TCGCTCT9800.033.0709724
TAATACT4950.032.0094344
TCTCGCT10750.030.8282282
CTAATCG950.00489120730.31589
TTCACCG6150.028.1058485
CCGTGTT6150.028.0975729
GTATTAG5950.027.8490281
GTGTTAC8600.027.6448761
GGGTTTC34250.027.5556011
CTCGCTC10750.027.4773333
TTGCTCT24200.027.3799724
ACCGTGT6750.026.6647138
GTTTCAC29350.026.019383
GTGTTAG14100.025.5475961
GTATAAC3501.4006218E-1024.7008781
CGCTCTG11100.024.6559185
Produced by FastQC (version 0.11.5)