FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037610
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8100.046.23571
TCTGTCG8100.043.546878
TCGCTCT9250.037.352884
TCTCGCT10800.036.6701552
GTATTAG7000.032.923881
TAGTACT3800.032.2025264
ATACCGT1352.2501983E-531.993626
CTGTCGC10450.031.687469
GTCTTGC19450.031.1041811
TTCACCG5150.030.7511485
CCGTGTT6550.029.6734719
GTCGTAA1000.00627589228.8083971
ACCGTGT6600.028.3579838
CGCTCTG11050.028.0125145
CTCGCTC12350.027.9768543
TATACTG5550.027.2378125
TAATACT5200.026.3011554
GGGTTTC29550.026.0786691
TCACCGT6350.026.073546
GTATTAC3659.094947E-1225.6513141
Produced by FastQC (version 0.11.5)