FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2298899
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8950.044.2429168
GCGTACG700.001090491841.1661341
CTGTCGC11250.039.0433929
GTCTCGC10450.032.4010961
TCGCACT2401.7007551E-930.0091344
GTCTTGC21900.029.2768251
GTCGTAA1255.351256E-428.8162941
TCGCTCT11250.027.528384
CGTACGG1050.00795333227.4440882
GTATTAT6100.027.16291
GGTATAC4700.026.0572851
GGGTTTC40800.025.0729981
CCGTTCG1450.001276783224.8297449
GTGTTAG16000.024.7641
CTCGCTC12000.024.6139183
TTACACT10450.024.1221754
ACCGTGT8150.023.8512028
GTTTCAC36000.023.613353
GTACGGA1851.9122798E-423.3645631
TCTCGCT12650.023.349172
Produced by FastQC (version 0.11.5)