FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2298899
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8650.044.104278
GTCTCGC9700.040.0922361
TCGCTCT11000.038.6048974
CTGTCGC10950.037.4698229
TCTCGCT12350.031.4798562
CTCGCTC12700.031.1703783
CGCTCTG12850.030.8092025
GTCTTGC21050.028.3964581
CTATCGA1306.763463E-427.685199
GTGTTAG16800.026.5778981
GTGTAAC7300.025.650081
GTCTTAC10000.025.2061351
GGGTTTC40650.023.7400971
GTTTCAC36750.023.1104163
GTATTAT7250.022.8469411
GTCTCAC30300.022.8174361
GTACTAT6400.022.5054781
GTACTAG4505.456968E-1222.4054531
TTCACCG7650.021.6414995
TAATACT7400.021.3980454
Produced by FastQC (version 0.11.5)