FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2134391
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8900.047.727038
GTCTCGC8650.040.815811
CTGTCGC11850.036.456599
GTCTTGC19900.035.8451651
TCGCTCT10700.033.6598744
TCTCGCT10800.033.357592
CTCGCTC10500.032.9382363
GTCTTAC7700.030.87961
GTATTAT5450.030.4074341
ACCGTGT6850.029.4286168
CGCTCTG12100.029.1700385
TACCCCG2253.0033334E-828.8128555
TAGTACT3800.028.4337394
TTCACCG5600.027.0120495
GTATAAC4050.026.6860731
GTGTTAC8750.026.3505921
GTATTCG1100.009970427526.2008721
GTACTAT5000.025.9388661
GGGTTTC33900.025.5052741
CCGTGTT8550.024.4216489
Produced by FastQC (version 0.11.5)