FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2134391
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9200.046.9445048
GTCTCGC9950.041.2579081
TCGCTCT11100.038.905314
CGCTCTG11300.038.8573075
CTGTCGC11400.037.8850369
GTTTACG1553.682544E-837.171811
GTCTTGC18900.036.962841
TCTCGCT12850.032.5003972
GTATAAC2757.2759576E-1231.4270761
CTCGCTC13600.029.6366943
TTGCTCT25900.028.901094
CTTGCTC24650.027.7387773
GTCGGAC1306.741985E-427.7001481
GTATTAG6750.026.6742171
GGGTTTC32000.025.8823241
TAATACT7000.025.7052974
GTGTAGT13900.025.3884811
GTCTCAC26500.025.2750781
GTTTCAC30750.024.8108353
GTCTAAC4101.8189894E-1224.5923251
Produced by FastQC (version 0.11.5)