FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993743
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8300.045.97218
GTCTCGC9500.042.4662861
TCGCTCT10700.040.385694
GTCTTGC17950.039.732931
CGCTCTG10450.037.2166635
TCTCGCT11450.035.8632132
CTGTCGC10700.034.9939049
TAGTACG1051.928006E-434.295784
CTCGCTC11900.033.296273
GTATAAT5600.028.3018261
GTATTAC4850.028.2222561
TTGCTCT28350.028.1987514
GTTTCAC29350.025.2818583
TTCACCG6300.025.1502385
TCTGTCA26150.025.0533758
CTTGCTC27250.024.8508453
GGGTTTC32900.024.5246031
TAATACT6600.024.0070464
GGTATAC4251.8189894E-1223.731161
TCTTGCT29850.023.6516552
Produced by FastQC (version 0.11.5)