FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874054
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8150.044.190291
TCGCTCT8700.039.7110944
TCTGTCG7450.038.6460578
TCTCGCT9650.036.5651442
GTCTTAC7350.033.320081
GTCTTGC17900.032.1922571
CTCGCTC9700.031.9061973
CTGTCGC9600.031.4904989
CGCTCTG10050.030.796685
TTATCCG950.00489897730.3058364
ACCGTGT6800.028.5796138
GTATTAT6400.028.1367861
TACACCG1804.9746213E-627.9915545
TTGCTCT23250.025.6947884
TATACTG5650.025.4790365
TTCACCG5450.025.0933465
GTGTAAA11950.024.1105181
GTGTAAT8750.023.8728561
GTGTTAG12900.023.4516831
CTTACTC9600.023.2417453
Produced by FastQC (version 0.11.5)