FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943595
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7450.044.4537128
TCGCTCT9250.039.7051354
CTGTCGC10100.037.7828339
GTCTTGC18450.037.094811
GTCTCGC8800.036.8396721
GTATAAC3950.031.0054281
CTCGCTC10800.029.3504543
TAATACT7650.029.1822344
GTATTAA6200.029.0492021
GTCTTAC8550.028.648291
CGCTCTG11650.028.4347975
TCTCGCT11700.027.0927282
TACCCGT1358.409264E-426.6719326
GTGTTAG13250.026.6419581
TTGCTCT26900.026.5033744
GTGTTAC9000.025.6149431
TTCACCG5350.025.5751445
TAGTACT4600.025.0484244
GTTAGCC11800.025.0315513
GGGTTTC33450.024.9831851
Produced by FastQC (version 0.11.5)