FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2054119
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8850.044.7715571
TCTGTCG9200.043.039998
TCGCTCT9000.038.4118424
TCTCGCT10850.034.5268062
CTGTCGC11000.034.0385749
GTGTTAC8650.031.6482931
CGCTCTG10600.031.2549215
CTCGCTC11550.029.9393253
GTCTTGC19100.029.0429081
GTATAAC3153.8198777E-1127.4443841
GTGTAGC9500.025.7832741
GGGTACG2001.1199467E-525.2145291
GTATTAT5450.025.1153871
GGGTTTC34000.024.790751
GTACCGT1751.3116481E-424.6999451
CCGTGTT7400.024.3259059
TTGCTCT27900.024.2655474
GTTAGCC11350.024.1196233
GTATTAG6350.023.8247491
GTCTCAC25250.023.680971
Produced by FastQC (version 0.11.5)