FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2054119
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9950.052.8087438
GTCTCGC9350.044.677291
CTGTCGC12500.042.611599
TCGCTCT10250.039.322264
TCTCGCT12050.035.254012
GTATTAT6000.033.6106761
CGCTCTG11600.033.5066685
CTCGCTC12150.033.1731033
GTCTTGC20600.030.7670521
GTATCGG1000.006275103428.8091471
GCCGTTA1000.00629379228.7916158
GTAACGC1555.7832163E-527.8608193
GTATTAG5700.026.5347441
GTATATA8200.026.3498331
GTATAGC4750.025.7766081
GTATTGG6150.025.7642781
GTCTTAC8400.025.7224561
GGCTAGA12950.024.471091
GTGTTAC8250.024.4441261
GTGTAAC8000.024.3077181
Produced by FastQC (version 0.11.5)