FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2078557
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8800.048.2947541
TCTGTCG9200.046.9530878
CTGTCGC11450.039.6166349
TCGCTCT10250.036.5329444
TCTCGCT11900.034.503132
CTCGCTC12200.031.292963
GTATACG1402.8721559E-530.8712231
TAATACT6100.030.6936634
GTCTTGC21050.029.7712971
CGCTCTG11800.029.2930415
ATTACGC1000.006270873428.8131433
TACGCTT1804.956846E-628.0046865
GTATTAC4700.027.5870511
GTATAGA5900.026.8597091
TATACGG1100.00998356126.1937662
GGATACG2508.345887E-825.9318261
GTCTTAC9650.025.3794521
GTATTAA5800.024.8389171
GTGTTAG14250.023.7582051
GCTATAC3652.382876E-1023.6820323
Produced by FastQC (version 0.11.5)