FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2080183
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9950.052.8094188
GTCTCGC10250.052.6910671
GCGTAAC859.3560993E-750.8313831
TCGCTCT11150.046.473634
CTGTCGC12600.043.9877559
TCTCGCT12650.043.2563172
CGCTCTG12500.040.8846175
CTCGCTC13600.040.2182963
GTCTTGC20600.038.1029741
TTGCTCT26150.031.9253224
GTATTAG6900.030.2655471
GTGTTAC8900.029.9372081
GGTATAC4900.029.3922981
CTTGCTC27500.027.4792963
TCTTGCT29800.026.09362
GTCTCAC23600.024.7156811
GTATAAC4700.024.5144271
GCTCTGT52550.024.2444426
GGGTTTC31500.023.77511
GTGTAAC6900.022.960071
Produced by FastQC (version 0.11.5)