FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2595433
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9150.035.418
GTCTCGC11050.033.2296451
GTCTTGC24900.028.6255361
GTATTAA8200.028.0966131
GTGTAAC7750.027.8700241
TAATACT8800.027.817244
GTGTTAC11300.027.3973031
CTGTCGC13450.027.3012439
TCGCTCT13450.027.300194
GTACAGG15350.025.7971731
GGGTACG2259.686919E-725.5991331
TCTCGCT14400.025.4991362
GTACGGG2001.12540765E-525.1991461
GTGTAAG13300.023.2774071
GTCTCAC30900.023.067181
GTGTTAG17600.022.9083161
CTCGCTC15500.022.760523
ACCGTTC2852.9742114E-722.7369488
GTGTTAA11300.021.6629831
GGTATAC6450.021.2085841
Produced by FastQC (version 0.11.5)