FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2842916
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11500.036.3107038
GTCTCGC13250.034.434031
CTGTCGC13900.032.630999
TCGCTCT14850.030.0613124
GTATTAA9200.029.912931
GTCTTGC27500.028.9683111
TCTCGCT16650.026.4124472
TAATACT10500.026.0577534
GTGTTAC12800.025.460431
CTCGCTC15900.025.391243
GTGTAAC10350.025.1898371
GTGTAGA17000.024.2822591
GTGTAGC13200.023.5916181
CGCTCTG15900.023.545655
TACACTG20150.023.2243085
GTATTAG10650.023.1202471
GTGTTAG18500.023.0963551
GGTATAC5750.022.670851
GTATAAC5800.022.4754141
GTACTAC5900.022.0944751
Produced by FastQC (version 0.11.5)