FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2459085
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9550.050.510048
GTCTCGC12900.042.661531
TCGCTCT12950.038.9181564
CTGTCGC12600.038.2834059
TCTCGCT15150.035.2070052
CTCGCTC15300.032.977413
CGCTCTG16000.029.6982125
GTATTAG10100.029.3951071
GTCTTGC22950.029.0280381
GTATTAA8400.027.5856511
GCGTAGA2207.572944E-726.3317581
GTCTTAC9600.024.891741
GTATTAT8150.024.877861
GTACCGC2101.6426968E-523.9985526
GTATATA12150.023.8394531
GTGTAAT10400.023.6732641
GTGTTAG17950.023.397861
GTGTTAC10450.022.8670541
GTGTAGG15200.022.8670541
GCGCGAT5450.022.4573619
Produced by FastQC (version 0.11.5)