FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2991573
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11750.031.2502148
GTCTTGC30200.027.653951
GTCTCGC13450.027.2994581
GTGTAAC9800.026.4473761
CTGTCGC14900.025.6100399
TCGCTCT14750.025.3815084
GTGTTAG21800.023.7783741
GGTATAC6250.023.0386031
CTCGCTC15800.022.7834263
GTATTAG9800.022.774131
GTATAAG9550.022.6164281
TCGTGCG1600.002265811222.4993889
GTATAGA10400.022.15251
GTACTAG7500.022.078661
GTATAAC6850.022.0716531
GTGTTAC13450.021.9466231
GTTTCAC46400.021.7228183
GGCGTAC2003.255056E-421.598691
GTGTAGT17700.021.5580141
GTCTAGC12400.021.4825671
Produced by FastQC (version 0.11.5)