FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2791618
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11700.041.8437778
GTCTCGC10750.039.737521
CTGTCGC13700.036.7862329
GTCTTGC26650.035.861951
TCGCTCT13350.031.8189854
TCTCGCT13800.029.767832
GTGTTAG20600.028.4691811
GTACGTA1855.8818496E-627.395771
CGCTCTG14050.026.6461725
CTCGCTC15750.026.082293
GTATAGC6200.025.6914251
TTGCTCT36650.025.5378884
GTCTTAC11900.024.9456061
GTATTAA8900.023.5920221
TCCGTAC2452.015875E-623.5328943
GTCCTAC7450.023.324491
GGGTTTC49050.023.3225081
GTATTAT10000.023.1689931
CCATGCG5950.022.990299
CTTGCTC37100.022.9223693
Produced by FastQC (version 0.11.5)