FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2791618
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11750.037.990328
GGTATAC6250.033.4063721
GTCTCGC11750.028.7985971
GTCTTGC25250.028.7985971
CTGTCGC15400.028.51869
TACACCG2054.2969623E-728.0966955
GTATTAT7500.026.8786871
GTATAGA9650.026.8587951
TCGCTCT12700.026.644374
GTATAAC5900.025.6258681
TTACACT14650.024.5721844
GATCGTT3001.8409992E-823.999269
GTATTAC6600.023.9988291
GTATTAG11400.023.3672831
GTGTTAG20050.023.3405081
GTGTAGG18300.022.818561
GTGTAAC9550.022.6166971
GTGTTAC12800.022.4989011
GTCTTAC11850.022.4799161
GTTTCAC44250.022.453143
Produced by FastQC (version 0.11.5)