FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2825188
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10200.045.8795288
TCGCTCT13800.038.6063164
CTGTCGC13150.037.2296459
GTCTCGC13800.034.6343271
CGCTCTG16400.032.0468065
GTGTTAC13300.030.4914671
TCTCGCT16800.029.1818642
GTCTTGC25550.028.9102041
GTATAGC6850.027.4868341
CTCGCTC17150.027.3251553
GTCTTAC11700.026.614881
GTATAAC6450.024.700451
TAATACT10000.024.4784934
GTGTAAA19250.024.4525721
GGGTTTC47350.022.6351641
TCTCGAT11200.022.4986137
GTATAAA12650.022.3262621
GTATAGA8450.022.2822251
TTCACCG7900.021.872075
GTATTAG10350.021.6901841
Produced by FastQC (version 0.11.5)