FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2825188
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11650.033.9914678
GTCTCGC11550.033.037841
CTGTCGC14550.028.7010739
TCGCTCT13000.028.2451974
GTCTTGC27050.027.9472961
ACGGTCG1050.00797555227.4286125
GTGTTAG20300.026.2454171
CGCTCTG14100.026.0425935
GTCTCAC34650.025.9731461
GTGTACG3701.0913936E-1125.2964381
CTCGCTC15100.024.7938613
TCTCGCT15200.024.1570762
TATACTG9850.024.1218625
GTATAAT9450.023.6182481
GTCTTAC13250.023.3652441
ATACCGT1851.919755E-423.3513876
GTAACGC1851.9202156E-423.3505573
GTATAAG8750.023.0392171
TTGCTCT40100.022.9817664
GTATTAC8150.022.9685461
Produced by FastQC (version 0.11.5)