FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2958431
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11400.042.944588
CTGTCGC14300.034.235549
GTCTCGC14950.033.9113581
TCGCTCT15700.033.47614
GTGTTAC13700.030.6616671
GTCTTGC29750.030.1872141
GCGTTAC1306.525348E-427.8557591
TCTCGCT18150.027.0099752
CGCTCTG17100.026.9456185
GTCTCAC31750.025.3202251
GTATAGT8950.025.0857431
CTCGCTC18500.024.939793
GTATTAA8450.024.8559071
GTGTTAG20700.024.8414151
GTATAAT9050.024.8085541
GTATTAG9750.023.7702451
GTGTTAT11600.023.1010681
GGTATAC6050.022.7450311
TTGCTCT38350.022.5282544
TTCACCG8950.022.5236765
Produced by FastQC (version 0.11.5)