FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2958431
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11750.038.6030858
GTCTCGC12950.037.2492031
TCGCTCT14550.035.6271824
GTATTAG10750.032.8170551
CCGATCG900.00375502331.9990279
CTCGCTC15650.031.2828663
CTGTCGC15600.030.9221369
TCTCGCT15700.030.2660832
GTCTTGC29500.029.5308071
GTGTAAT14000.028.2843761
CGCTCTG16700.028.02315
GTCTTAC11200.026.9987221
GTATAGT8550.025.2619631
TACACCG2301.1732991E-625.0427195
GGCGTAC2151.970761E-523.4407521
ACCGTGT10800.023.3326248
GTGTTAC14150.022.8964421
CATACCG1902.3003315E-422.736155
TTACACT15400.021.9729864
GTATTAA8550.021.8937021
Produced by FastQC (version 0.11.5)