FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2750760
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12600.043.99761
TCTGTCG12100.039.8670438
TCGCTCT15550.037.502784
CGCTCTG17100.034.525695
GTCTTGC25800.032.6493841
CTGTCGC14900.032.375259
CTCGCTC16750.031.3773943
TCTCGCT17000.031.3394662
GTATTAG9650.029.8429321
GCGTAAC1555.7717283E-527.869451
CCGTGTT9850.025.583299
ACCGTGT9650.025.3674148
GTATTAT10350.025.0421121
GTGTTAC11250.024.958641
GTATTCG2051.3630461E-524.5840261
GTACTAG5150.023.7656961
GGGTTTC46500.023.689031
GTCTCAC33050.023.5267051
GTCTTAC11550.023.0636771
TTCACCG8500.022.8701735
Produced by FastQC (version 0.11.5)