FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2556116
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1004.97148E-850.399275
TCTGTCG11500.048.2079938
GTCTCGC12400.043.5469061
CTGTCGC14050.041.5082939
TCGCTCT14200.037.012824
GTCTTGC22700.034.888681
CGCTCTG15550.030.5590425
TCTCGCT16450.030.199582
CTCGCTC16250.029.2420793
TTCACCG7350.028.4077515
GTATTAC5650.028.0344431
GTATAGA8400.027.4276371
AGTACCG1358.4204087E-426.6662795
ACCGTGT9050.026.2537638
GTATAAC4950.026.1809231
GTCTTAC10750.026.1200391
CCGTCTA2001.1252301E-525.1996359
GTCTCAC30000.024.959151
GGTATAC5800.024.8267381
TTGCTCT33500.024.2857384
Produced by FastQC (version 0.11.5)