FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085783
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9300.040.257168
GTCTCGC9450.033.6173441
GTCTTAC7900.030.1598641
CTGTCGC11550.029.9214069
GTCTTGC20800.029.505171
TAATACG1504.614337E-528.7993534
TCGCTCT10750.028.12964
GTATTAG6750.027.8107131
TCTCGCT11650.026.5757852
TAATACT5750.025.0429154
TCTCGAT7550.024.7941467
CTCGCTC13150.024.091873
GTGTAAT8950.023.3947011
GTGTTAC9900.023.3376621
GTGTTAG14950.023.1815551
TCGATCT7250.022.8408669
CGCTCTG12700.022.6766555
GTATTAA6400.022.5627781
TTGCTCT28100.022.5475354
GTCTCGA17750.022.3093576
Produced by FastQC (version 0.11.5)